Custom Protein Expression

🧪 Looking for custom recombinant protein expression support?

At PawThNeo, we specialize in rapid, reliable protein production in E. coli, Pichia, insect, and mammalian systems tailored for drug discovery, diagnostics, vaccine development, and enzyme R&D.

We offer:

✅ Gene-to-protein workflows

✅ Soluble & refolded protein expression

✅ Strain Development

➡️ Looking to express your next protein construct? Let’s connect!

📩 info@pawthneo.com

Informatics

Data mining, analysis, and recommendations about protein targets for applications in healthcare and sustainable solutions

Customized Protein Production for the following applications:

Protein targets for Small molecule Drug Discovery
Biologics or Biosimilars
Proteins for Diagnostics
Protein based Antigens
Biocatalysts
Smart proteins including growth factors

Construct Design: Laying the Foundation for Success

We engineer precise DNA constructs tailored to your needs
In Silico design: Identification of unannotated genes, selection of relevant domains, inclusion or exclusion of purification or solubility tags, selection of host/s for high-level expression.
Gene Synthesis & Optimization: Custom gene design with codon optimization for enhanced expression
Vector Selection: Choose from our exhaustive library or request custom vectors for expression in recombinant hosts
Validation of expression constructs: Sanger sequencing and bioinformatics analysis to ensure accuracy

Recombinant expression: Unlocking Host potential

Leverage our platforms for scalable, high yield protein production

Host systems: E. coli, Pichia pastoris, Baculovirus expression vector systems (BEVS), mammalian cell lines*
Expression elements: Promoters (constitutive/inducible), episomal or integrated, desired selectable markers
Tags: Solubility and purification tags or devoid of tags
Site of expression: Inclusion body/ periplasm (E. coli), Cytoplasm (All), Secretion (Pichia pastoris, insect cells, mammalian cells)
Co-expression: Co-expression with interacting proteins or chaperones/ disulfide isomerases/ other helper proteins

Purification: Discarding the impurities

Fractionation

Refolding from inclusion bodies
Periplasmic extraction
Processing culture supernatant and cytoplasmic fraction

Chromatography

Affinity, ion exchange, and size exclusion chromatography [Fractionation of oligomers shown in the imge below]

Optimization of Scalable processes

Additional capabilities

Characterization of Proteins
- Cell-free assays- Non-Native & Native (Biochemical and Biophysical assays)
- Cell-based assays
Monoclonal antibody production
Access to Fermenters

For service inquiries, please write to info@pawthneo.com

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